1. BAP1 is required for homeostatic and antigen-driven expansion of peripheral T cells.
BAP1 is required for homeostatic and antigen-driven expansion of peripheral T cells. (A) Sorted CD4+ T cells from Bap1fl/flRosa26CreERT2 or Bap1wt/wtRosa26CreERT2 mice 1 week after tamoxifen injection were transplanted into Rag2−/− recipients either separately or at a 1:1 ratio. Numbers of total splenic CD4+ T cells in recipient mice 3 weeks after transfer (n = 3 to 4) are shown. Data are representative of two independent experiments. Error bars represent SD. *P < 0.05, **P < 0.01 based on unpaired Student’s t test. (B) GSEA of WT versus KO nonpolarized, TH2, and TH17 data sets. Gene set–level heat maps (left) and FDR (right) of gene sets that are differentially enriched in WT versus KO with an FDR less than 20% are shown. (C) Number of cells recovered after 6 days of culture of naïve CD4 T cells from Bap1fl/flCd4-Cre+ (red) and Bap1wt/wtCd4-Cre+ (black) mice stimulated with anti-CD3 and anti-CD28 (n = 2). (D) Cell cycle analysis of naïve CD4 T cells from Bap1fl/flCd4-Cre+ or Bap1wt/wtCd4-Cre+ mice stimulated with anti-CD3 and anti-CD28 for 1 or 2 days (left) and proportion of cells in each phase of the cell cycle (right) (n = 3) are shown. ****P < based on Fisher’s exact test. (E) CellTrace-labeled naïve CD4 T cells from Bap1fl/flCd4-Cre+ or Bap1wt/wtCd4-Cre+ mice were stimulated with anti-CD3 and anti-CD28 for the indicated number of days (n = 8). CellTrace profiles (left) and number of live cells recovered (right) are shown. ****P < based on unpaired Student’s t test. 7-AAD, 7-aminoactinomycin D.
Teresita L. Arenzana et al. Sci. Immunol. 2018;3:eaal1953
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