1. Association of peroxisomes with microtubules is required for proper distribution but not division.
Association of peroxisomes with microtubules is required for proper distribution but not division. (A) AOx-deficient fibroblasts were cultured for 4 hours in the absence (Aa,Ab) or presence of 150 μM DHA (Ac–Af) followed by the addition of DMSO (Aa–Ad) or 10 μM nocodazole (Ae,Af) for 20 hours. Cells were stained with antibodies to Pex14p (Aa,Ac,Ae) and α-tubulin (Ab,Ad,Af). Arrows indicate peroxisomal clusters. Scale bars: 20 μm. (B) Microtubules are not involved in peroxisomal elongation. AOx-deficient fibroblasts were treated with 10 μM nocodazole for 1 hour and then cultured for 12 hours in the presence of 150 μM DHA. Cells were stained with antibodies to Pex14p (Ba) and α-tubulin (Bb). Insets are higher-magnification images of the boxed regions. Scale bars: 10 μm and 2 μm (inset).
Akinori Itoyama et al. J Cell Sci 2012;125:
© 2012.