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Ryan K. C. Yuen, Ph. D. , Anna Merkoulovitch, B. CS. , Jeffrey R

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Presentation on theme: "Ryan K. C. Yuen, Ph. D. , Anna Merkoulovitch, B. CS. , Jeffrey R"— Presentation transcript:

1 Development of a high-resolution Y-chromosome microarray for improved male infertility diagnosis 
Ryan K.C. Yuen, Ph.D., Anna Merkoulovitch, B.CS., Jeffrey R. MacDonald, B.Sc., Matthew Vlasschaert, B.Sc., Kirk Lo, M.D., Ethan Grober, M.D., Christian R. Marshall, Ph.D., Keith A. Jarvi, M.D., Elena Kolomietz, M.D., Ph.D., Stephen W. Scherer, Ph.D.  Fertility and Sterility  Volume 101, Issue 4, Pages e3 (April 2014) DOI: /j.fertnstert Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Graphical displays of structural variation detected by microarray on the Y chromosome. The examples displayed include (A) an AZFa microdeletion, (B) an AZFc microdeletion, (C) an AZFb+c microdeletion, and (D) a structural rearrangement with Yp duplication and Yq deletion. Probes with positive, negative, and balanced log ratio values are colored as blue, red, and green, respectively. The shaded areas represent the regions where variants were detected. Fertility and Sterility  , e3DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

3 Supplemental Figure 1 Distribution of probes along the Y chromosome. (A) Location of different AZF deletions (shaded regions). (B) Density of the probes across the Y chromosome. (C) Corresponding loci of the genes/STS markers. Fertility and Sterility  , e3DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

4 Supplemental Figure 2 Hierarchical clustering of 31 samples with deletions/rearrangements detected and 31 samples with no deletion detected on the Y chromosome. Clustering was performed by Pearson’s correlation of 2,274 probes, which were selected based on their differential log ratio values between sample groups (P<.05; Student’s t-test). Fertility and Sterility  , e3DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions


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