1. Apoptosis and expression of apoptosis-related genes in the mouse testis following heat exposure 
Michiharu Miura, M.D., Isoji Sasagawa, M.D., Yasuhiro Suzuki, M.D., Teruhiro Nakada, M.D., Junichi Fujii, Ph.D. 
Fertility and Sterility 
Volume 77, Issue 4, Pages (April 2002)
DOI: /S (01)

2. FIGURE 1
In situ end labeling of DNA fragmentation on testis sections. (A), Testis before heat exposure. Few in situ-labeled germ cells are observed in the seminiferous epithelium. (B), Testis from 1 day after heat exposure. Labeled cells are frequently found in the seminiferous epithelium. Magnification ×200. Arrows indicate apoptotic cells.
Miura. Testicular germ cell apoptosis by heat exposure. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01) )

3. FIGURE 2
Quantitative analysis of changes in Bcl-xl, Bcl-2, Bad, and Bax gene products in the testis after heat exposure. Upper panels: Quantitative densitometric analysis of gene products was determined on Western blots at the time points indicated. Each data point represents mean ± SD (n = 4). Data are presented as percentage of day 0, with day 0 values having been assigned a value of 100%. OD: optical density. ∗P<.05 compared with day 0. Lower panels: Western blot analysis was performed using testis samples obtained from mice before heat exposure and 1, 2, 3, 7, 14, 21, 28, 42, and 56 days after heat exposure. The gels are representative of one animal at each time point. The molecular sizes of the bands shown on the left of the figure were determined by markers run on the same gel.
Miura. Testicular germ cell apoptosis by heat exposure. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01) )

4. FIGURE 3
Upper panels: Quantitative analysis of changes in FasL, Fas, and p53 expression in the testis after heat exposure. Quantitative densitometric analysis of gene products was determined on Western blots at the time points indicated. Each data point represents mean ± SD (n = 4). Data are presented as percentage of day 0, with day 0 values having been assigned a value of 100%. OD: optical density. ∗P<.05: compared with day 0. Lower panels: Western blot analysis was performed using testis samples obtained from mice before heat exposure and 1, 2, 3, 7, 14, 21, 28, 42, and 56 days after heat exposure. The gels are representative of one animal at each time point. The molecular sizes of the bands shown on the left of the figure were determined by markers run on the same gel.
Miura. Testicular germ cell apoptosis by heat exposure. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01) )