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IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. Protein (10–20 μg) was separated by SDS-PAGE (6% polyacrylamide gels). Semiquantification was performed as shown in Fig. 1. Results for each cell line are expressed as the mean ± SD of 6–9 cell lysates from individual experiments (D-NOD vs. ND-NOD mesangial cell lines, ***P < 0.001). Ivan Tack et al. Diabetes 2002;51: ©2002 by American Diabetes Association
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