1. M. D. Jasani, J. H. Kamdar, A. K. Maurya and S. K. Bera
QTL MAPPING FOR RESISTANCE TO PEANUT BUD NECROSIS DISEASE (PBND) IN PEANUT (Arachis hypogaea L.)
M. D. Jasani, J. H. Kamdar, A. K. Maurya and S. K. Bera
ICAR-Directorate of Groundnut Research,
Junagadh, Gujarat.

2. Peanut Peanut is an economically important oilseed
crop cultivated in an area of ~25.44 m ha,
with a production of ~45.22 m tons
Peanut is a valuable source of edible oil
(48–50%) and protein (26–28%) for human beings and fodder for livestock
India alone produces ~ 20% (9.47 m tons) of total peanut production in the world
Gujarat ranked first in area and production and produces more then 50% of total peanut production in India

3. Peanut Bud Necrosis Disease
PBND is caused by Peanut bud necrosis virus (PBNV), vectored by Thrips palmi Karny
PBND has emerged as a serious yield
constraint of peanut especially in South Asia
and in parts of China, Nepal, Sri Lanka and
Thailand
It can cause yield losses up to 70-80% in peanut and many other crops
Thus, it is very important to identify markers linked to PBND resistance for widening the genetic base of cultivated peanut.

4. Symptoms of PBND
Infected

5. Methodology Plant Material JL 24 : susceptible to PBND
NRCGCS 85 : resistant to PBND
Hybridization and generation advancement
108 RILs were derived from cross between JL 24 x NRCGCS 85 by selfing of F1 seeds
Phenotyping and genotyping
Phenotyping of RILs - UAS, Raichur, Karnataka
Genotyping of RILs - polymorphic SSRs markers
Linkage and QTL mapping
Genetic linkage groups and QTL mapping were constructed using the statistical software QTL IciMapping V4.1

6. Result
Phenotyping
Phenotyping of 108 RILs with both parents was done under natural field conditions in the field of UAS, Raichur, Karnataka during Rabi 2017.
Frequency distribution for PBND incidence

7. Genotyping
A total of 1980 SSRs were used in polymorphism survey, 227 (11.46%) SSRs were found polymorphic
Among them only 49 SSRs found co-dominant which were further used in linkage analysis and QTL mapping

8. Linkage mapping
A genetic linkage map comprising of 47 SSRs on 8 linkage groups with total genome distance of cM.
The longest linkage group was LG6 (201.61cM) and shortest was LG5 (20.27cM).

9. Linkage map prepared using 47 SSRs in peanut

10. Single marker Analysis
QTL mapping
Single marker Analysis

11. Composite Interval mapping
Two major QTLs were identified for resistance to PBND on linkage group six and seven.
QTLPBND-01 is flanked by IPAHM 509 and AHS0006 and QTLPBND-02 is flanked by TC4G10 and TC9C08.
LOD score and phenotypic variation ranged from 3.13 to 3.62 and to 16.88, respectively.
Trait
QTL name
Linkage Group
Position (cM)
Marker interval
LOD
PVE (%)
Additive effect
PBND
QTLPBND-01 6
29.00
IPAHM AHS0006
3.62
16.88
2.90
QTLPBND-02 7
80.00
TC4G TC9C08
3.13
12.38
2.47

12. Location of QTL for resistance to PBND

13. Conclusion
To our understanding this is the first report on QTLs for PBND resistance in peanut
The identified linked SSRs (IPAHM509, AHS0006, TC4G10, TC9C08) may be used in peanut breeding programme for developing PBND resistance peanut varieties after validation in different background

14. Thank you