1. Quantification of Melanoma Micrometastases in Sentinel Lymph Nodes Using Real-Time RT-PCR 
Thomas Giese, Monika Engstner, Ulrich Mansmann, Wolfgang Hartschuh, Bernhard Arden 
Journal of Investigative Dermatology 
Volume 124, Issue 3, Pages (March 2005)
DOI: /j X x
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Quantification of Melan-A transcripts in comparison with histopathology. Melan-A transcripts per μL of real-time RT-PCR from sentinel lymph node (SLN) that were positive (triangles) or negative (circles) by histopathology. SLN positive by hematoxylin and eosin (HE) staining (n=21) are shown as open triangles, SLN that were HE negative but immunohistology positive (n=6) are shown as solid triangles. Median transcript numbers (horizontal lines) were 696 (8.2–3950) and 4.0 (2.2–7.9) transcripts per μL (lower and upper quartiles in brackets) for histopathologically positive (n=27) and negative (n=32) SLN, respectively. Melan-A transcript numbers were significantly different between the SLN groups defined by positive and negative histopathology (p<0.0001).
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Kaplan–Meier disease-free survival. Probability of recurrence-free survival for patients whose sentinel lymph node (SLN) were histopathologically and real-time RT-PCR positive (Path+/PCR+), for patients with histopathologically negative SLN who were reclassified by positive PCR (Path-/PCR+), and for patients with SLN negative by both assays (Path-/PCR-).
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions