1. Molecular Biotechnology 4C 28-11-2018 1 2 3 4 x MWM* *
Please confirm the order of
application in the gel
1 – Bam HI
2 – Hind III
3 – Eco RI
4 –PstI
X – do not consider
MWM–Molecular weight marker *
* hybridization signal
Since there is no specific hybridization signal in all the wells of this membrane, use for your report the “reference membrane “ for the comparison with the in silico analysis.
Do not forget to compare this result with the gel of the restriction of genomic DNA – try to explain why there is no signal in lane 4