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Published byBřetislav Moravec Modified over 5 years ago
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Tcf4 is highly expressed and Tcf4GFPCre+neo genetically labels muscle connective tissue fibroblasts in culture. Tcf4 is highly expressed and Tcf4GFPCre+neo genetically labels muscle connective tissue fibroblasts in culture. (A-D) Adherent fibroblasts derived from neonatal limb muscles express Tcf4 and αSMA (arrows, A-C) and vimentin (D). (E-G) In a preparation of adherent cells from limb muscles from Tcf4GFPCre+neo/+;R26RYFP/+ mice, 58% of Tcf4+ fibroblasts are genetically labeled with YFP (arrows, E-G). (H-J) In a preparation of total cells from limb muscles from Tcf4GFPCre+neo/+;R26RYFP/+ mice, MyHC+ myogenic cells are not labeled by YFP. Scale bar: in J, 100 μm for A-J. Sam J. Mathew et al. Development 2011;138: © 2011.
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