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NoCut: Cytokinesis in Check

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Presentation on theme: "NoCut: Cytokinesis in Check"— Presentation transcript:

1 NoCut: Cytokinesis in Check
Kerry Bloom  Cell  Volume 125, Issue 1, Pages (April 2006) DOI: /j.cell Copyright © 2006 Elsevier Inc. Terms and Conditions

2 Figure 1 Cytokinesis Gone Awry
The Lac repressor, a DNA binding protein, fused to GFP binds to lac operator sequences integrated into the yeast genome. This fluorescently labels a region of the yeast DNA (visualized as a green spot) that can be monitored using timelapse video microscopy. The outlined cell contains a lagging dicentric chromosome that fails to segregate into both mother (M) and daughter (D) cells (panel 1, leftmost panel). Upon cell separation (noted by change in position of the daughter and mother cell between the first and second panel), DNA left in the cytokinesis furrow is extruded from the cell (panel 2) and released from these cells over time (panels 3 and 4). Images courtesy of Doug Thrower and Kerry Bloom. Cell  , 17-18DOI: ( /j.cell ) Copyright © 2006 Elsevier Inc. Terms and Conditions

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