1. Quantitative evaluation of isolated Calliphora rhabdoms labelled for Rh3, Rh5 and Rh6.
Quantitative evaluation of isolated Calliphora rhabdoms labelled for Rh3, Rh5 and Rh6. Isolated Calliphora rhabdoms were double labelled with antibodies specific for Rh5 and Rh6 (A,B) or with antibodies against Rh3 and Oregon Green-coupled wheat germ agglutinin (C,D). (A) An overlay of differential interference contrast and indirect immunofluorescence images of two rhabdoms, one labelled with the anti-Rh5 antibody (red) and the other one labelled with the anti-Rh6 antibody (green). (B) The percentage of rhabdoms containing Rh5 or Rh6 was determined by counting the number of rhabdoms stained with either antibody. (C) A few rhabdoms viewed with differential interference contrast optics, one of which is labelled with anti-Rh3 (red). (D) The percentage of rhabdoms stained with anti-Rh3 was determined relative to the total number of rhabdoms stained with wheat germ agglutinin. For the quantitative evaluation, four independent preparations from both male and female flies were analysed. Values are means ± s.d. Scale bar, 40 μm.
Angelika Schmitt et al. J Exp Biol 2005;208:
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