1. Expression of candidate neuronal IEGs in the brain of cycloheximide pretreated crickets 30 min after PTX injection.
Expression of candidate neuronal IEGs in the brain of cycloheximide pretreated crickets 30 min after PTX injection. A, PTX-induced neuronal hyperexcitability in the cricket. Crickets show seizure-like behavior ∼2 min after PTX injection. 1 h before PTX/vehicle injection, 20 mM cycloheximide was injected to block de novo protein synthesis. B–G, Expression of (B) Gryllus fra total transcript, (C) fra-A isoform, (D) fra-B isoform, (E) jra, (F) egr-B, and (G) hr38 in the brains of cycloheximide pretreated crickets 30 min after injection of vehicle (5% DMSO in saline) or PTX. Expression levels of each target gene were normalized with that of Gryllusef1α gene (Fig. 1-1). RT-qPCR analyses were performed on eight biological replicates. Box plots indicate the 25th to 75th percentile ranges and central values. Error bars indicate the 5th to 95th percentile ranges. The “+” denotes the mean. Asterisks denote statistical significance (*, p < 0.05). See Table 3 for the details of statistical analysis. See Figs. 1-2, 1-3, 1-4, and 1-5 for the structures of the encoded proteins of candidate neuronal IEGs.
Takayuki Watanabe et al. eNeuro 2018;5:ENEURO
©2018 by Society for Neuroscience