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Intranasal administration of eotaxin increases nasal eosinophils and nitric oxide in patients with allergic rhinitis  Toyoyuki Hanazawa, MD, PhD, Julio.

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Presentation on theme: "Intranasal administration of eotaxin increases nasal eosinophils and nitric oxide in patients with allergic rhinitis  Toyoyuki Hanazawa, MD, PhD, Julio."— Presentation transcript:

1 Intranasal administration of eotaxin increases nasal eosinophils and nitric oxide in patients with allergic rhinitis  Toyoyuki Hanazawa, MD, PhD, Julio D. Antuni, MD, Sergei A. Kharitonov, MD, PhD, Peter J. Barnes, DSc, FRCP  Journal of Allergy and Clinical Immunology  Volume 105, Issue 1, Pages (January 2000) DOI: /S (00) Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 Effect of nasal lavage on nasal NO level. There was no significant difference between 6 normal control subjects and 6 patients with allergic rhinitis, and all these changes were within 10% over the baseline. Nasal NO had a tendency (P < .1) to increase in normal subjects at 2 hours after nasal lavage compared with that in patients with allergic rhinitis. Values are means ± SEM. Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 The dose-response relationship of eotaxin challenge and nasal NO. Eotaxin induced a clear dose-dependent increase in nasal NO, and the maximum change of nasal NO was observed at 8 hours after the challenge of 8 μg of eotaxin. Values are means ± SEM (n = 3). Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 A, Symptom scores after eotaxin and diluent (PBS-0.3% HSA) challenge. There were no symptoms after diluent challenge. Eotaxin caused a significant increase (P < .05) in symptom scores at 8 hours after challenge. B, Effect of eotaxin challenge on nasal NO level. There was no significant increase in nasal NO after diluent challenge. Nasal NO was increased significantly (P < .05) at 2, 4, 8, and 24 hours after eotaxin challenge compared with diluent challenge. The maximal increase in nasal NO (34.8% ± 5.5%) was observed 8 hours after eotaxin challenge. Values are means ± SEM (n = 9). Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

5 Fig. 4 Effect of eotaxin challenge on nasal lavage cells. The diluent challenge did not induce a significant influx in any cells at any time points. Eosinophils were increased significantly (P < .05) at 8 hours and returned to baseline levels 24 hours after eotaxin challenge. We found no significant difference in the influx of neutrophils and macrophages at any point after eotaxin challenge. On the other hand, there was a slight trend toward an influx of basophils and lymphocytes at 8 hours and of epithelial cells 24 hours after eotaxin challenge, but these changes were not statistically significant. Values are means ± SEM (n = 9). Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

6 Fig. 5 iNOS immunoreactivity in nasal lavage cells before (A) and 8 hours after (B) eotaxin challenge. Immunoreactivity of iNOS was found mainly in epithelial cells (arrows) and eosinophils (arrowheads) before and 8 and 24 hours after eotaxin challenge. However, it was difficult to estimate the difference between iNOS protein expression before and after the challenge (bar = 20 μm). Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

7 Fig. 6 Nitrotyrosine immunoreactivity in nasal lavage cells before (A) and 8 hours after (B) eotaxin challenge. Nitrotyrosine immunoreactivity was seen in eosinophils (arrowheads) and remained unchanged after eotaxin challenge. Levels of immunoreactivity for nitrotyrosine in epithelial cells (arrows) were low before eotaxin challenge but were moderately increased after eotaxin administration (bar = 20 μm). Journal of Allergy and Clinical Immunology  , 58-64DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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