1. Effect of optogenetic inhibition of D2-MSNs or D1-MSNs in the VLS on goal-directed behavior in mice.
Effect of optogenetic inhibition of D2-MSNs or D1-MSNs in the VLS on goal-directed behavior in mice. A, Illumination site in the bilateral VLS. Scale bar, 1 mm. B, C, The timing of illumination in the PR task. B, Yellow or blue light was applied at the time of every TS with the lever presentation for 2 s (yellow bar). C, Yellow or blue light was applied at the time of LP behavior for 2 s (yellow bar). D, The effect of yellow-light illumination at the time of TS (B) in D2-ArchT (top row) and D1-ArchT (bottom row) mice, respectively. Yellow-light illumination decreased breakpoint (1), prolonged first LP latency (2), and time spent to complete the PR (3) in both D2 and D1-ArchT mice, whereas it did not affect the active LPs (4), percentage inactive LPs (5), or collect reward latency (6). E, The effect of yellow-light illumination at the timing of LP (C) in D2-ArchT (top row) and D1-ArchT (bottom row) mice, respectively. Yellow-light illumination had no effect in D2-ArchT mice, whereas it decreased breakpoint (1) and active LPs (4) in D1-ArchT mice. Other behavioral parameters were not affected by yellow-light illumination in D1-ArchT mice. *p < 0.05, compared with the blue light control (paired t test). n = 12 sessions from 6 D2-ArchT mice; n = 8 sessions from 3 D1-ArchT mice. Error bars indicate + SE.
Akiyo Natsubori et al. J. Neurosci. 2017;37:
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