1. Subcellular localization of VAN3.
Subcellular localization of VAN3. (A) Overview of the functional VAN3-VENUS fusion construct used. The positions indicated are relative to the translational start site. The VENUS tag was translationally fused to the 3′ end of the ORF of the genomic XbaI-SpeI fragment. (B-G) Localization of GFP-tagged organelle markers (green) and VENUS-tagged VAN3 (red) in young leaf petioles. Localization of the TGN marker GFP-SYP41 (B), VAN3-VENUS (C) and their merged image (D). Merged images of VAN3-VENUS with GFP-SYP41 (E), with the Golgi body marker ST-GFP (F) and with the PVC marker GFP-ARA7/RAB-F2b (G). The inset of E indicates an enlarged view of the TGN-localized VAN3-VENUS. The arrowheads show the TGN-localized VAN3-VENUS and the arrows show the VAN3-VENUS signal that is not marked by GFP-SYP41. Scale bars: 10 μm.
Satoshi Naramoto et al. Development 2009;136:
© 2009.