1. PTX-induced reporter protein expression in the brain of the IEG reporter line.
PTX-induced reporter protein expression in the brain of the IEG reporter line. Distribution of the reporter protein (EYFPnls:PEST) in the brain of the IEG reporter line was examined by whole-mount fluorescent immunohistochemistry. A, B, Frontal views of the supraesophageal ganglion stained with anti-GFP antibody. A, EYFP immunoreactivity was only observed in the cells is indicated by white arrowheads 6 h after vehicle injection. B, EYFP immunoreactivity was observed throughout the ganglion 6 h after PTX injection. C, D, Ventral views of the subesophageal ganglion stained with anti-GFP antibody. C, EYFP immunoreactivity was not observed 6 h after vehicle injection. D, EYFP immunoreactivity was observed throughout the ganglion 6 h after PTX injection. Dorsoventral (D-V) or rostrocaudal (R-C) axes were indicated. Scale bars represent 200 µm. See Movies 1 and 2 for the full stack of optical sections of the supraesophageal ganglia shown in A and B.
Takayuki Watanabe et al. eNeuro 2018;5:ENEURO
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