1. Morphological segmentation of yeast by image analysis
Authors: Marco A.G. de Carvalho a, Roberto de A. Lotufo a, Michel Couprie
Source:Image and Vision Computing Volume 25, Issue 1, January 2007, Pages 34-39
speaker: Kuo Hua Wang
Date: 2007/11/15

2. Outline Introduction Watershed transform Minimum Spanning Tree
Tree of Critical Lakes (TCL)
Results
Conclusions

3. Introduction
Image segmentation

4. Introduction
Histogram

5. Watershed transform

6. Watershed transform

7. Minimum Spanning Tree 6 1 8 5 2 10 3 4 7 9
Construct MST by the centre of gravity of every region.

8. Tree of Critical Lakes (TCL)

9. Tree of Critical Lakes (TCL)

10. Results
80<ST<500

11. Results

12. Conclusions
presented a method to segment yeast cells based on hierarchical scale-space analysis.