Presentation is loading. Please wait.

Presentation is loading. Please wait.

IgE induces transcriptional regulation of thymic stromal lymphopoietin in human airway smooth muscle cells  Naresh Singh Redhu, MSc, Ali Saleh, PhD, Hai-Chon.

Published byTracy Jackson Modified over 5 years ago

Similar presentations

Presentation on theme: "IgE induces transcriptional regulation of thymic stromal lymphopoietin in human airway smooth muscle cells  Naresh Singh Redhu, MSc, Ali Saleh, PhD, Hai-Chon."— Presentation transcript:

1 IgE induces transcriptional regulation of thymic stromal lymphopoietin in human airway smooth muscle cells  Naresh Singh Redhu, MSc, Ali Saleh, PhD, Hai-Chon Lee, PhD, Andrew J. Halayko, PhD, Steven F. Ziegler, PhD, Abdelilah S. Gounni, PhD  Journal of Allergy and Clinical Immunology  Volume 128, Issue 4, Pages e2 (October 2011) DOI: /j.jaci Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 TSLP mRNA (A) and protein expression (B) were measured from ASM cells stimulated with IgE or TNF-α by using real-time RT-PCR and ELISA, respectively. C, ASM cells were transiently transfected with human TSLP promoter luciferase construct and stimulated with IgE or TNF-α in the presence of a Syk inhibitor, R112, or vehicle (dimethyl sulfoxide). TSLP promoter activity was measured. n = 4, ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 compared with the unstimulated (Fig 1, A, Mann-Whitney test; Fig 1, B, unpaired t test) or stimulated (Fig 1, C, unpaired t test) control. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 A, Lentiviral transduction efficiency in ASM for the Syk-shRNAs as determined by FACS by using turbo-green fluorescent protein as a marker. B, Lentiviral Syk-shRNA transduction silenced the ASM Syk expression efficiently as observed by Western blotting. C, Syk-silenced and scramble ASM cells were transfected with a TSLP promoter construct, stimulated with IgE or TNF-α for 12 hours, and TSLP promoter activity was measured. n = 3, ∗P < .05, ∗∗P < .01 (Fig 2, C, unpaired t test) compared with the unstimulated control. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Primary ASM cells were transfected with (A) mutant constructs of NF-κB (M1), AP-1 (M2), or WT TSLP promoter (WT); (B) WT TSLP promoter construct and NF-κB/p65 subunit overexpressing plasmid; and (C) WT TSLP promoter and dnIKKβ. Cells were stimulated with IgE or TNF-α for 12 hours, and luciferase reporter activity was measured. n = 3, ∗P < .05, ∗∗P < .01 (Fig 3, A, Mann-Whitney test; Fig 3, B and C, unpaired t test) compared with the stimulated WT control. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "IgE induces transcriptional regulation of thymic stromal lymphopoietin in human airway smooth muscle cells  Naresh Singh Redhu, MSc, Ali Saleh, PhD, Hai-Chon."

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,
Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 

Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 
Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F

Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F
An IgE-associated polymorphism in STAT6 alters NF-κB binding, STAT6 promoter activity, and mRNA expression  Michaela Schedel, PhD, Remo Frei, PhD, Christian.

An IgE-associated polymorphism in STAT6 alters NF-κB binding, STAT6 promoter activity, and mRNA expression  Michaela Schedel, PhD, Remo Frei, PhD, Christian.
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.

Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.
Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,

Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,
Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.

Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.
Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,

Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,
Regulation of GM-CSF expression by the transcription factor c-Maf

Regulation of GM-CSF expression by the transcription factor c-Maf
Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 

Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 
Elena Goleva, PhD, Leisa P. Jackson, BS, Melanie Gleason, PA, Donald Y

Elena Goleva, PhD, Leisa P. Jackson, BS, Melanie Gleason, PA, Donald Y
Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.

Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.
Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,
IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
Differences in human gingival and dermal fibroblasts may contribute to oral-induced tolerance against nickel  Lina Gölz, MD, Elisa Vestewig, Moritz Blankart,

Differences in human gingival and dermal fibroblasts may contribute to oral-induced tolerance against nickel  Lina Gölz, MD, Elisa Vestewig, Moritz Blankart,
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Jennifer L. Ingram, PhD, Monica Kraft, MD 

Jennifer L. Ingram, PhD, Monica Kraft, MD 
Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA  Hirokazu Kinoshita, MD,

Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA  Hirokazu Kinoshita, MD,

Similar presentations

Ads by Google