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Chd1 expression and CHD1 localization during mouse preimplantation development.
Chd1 expression and CHD1 localization during mouse preimplantation development. (A) qRT-PCR analysis of Chd1 mRNA expression during mouse preimplantation development. Expression levels at each developmental stage were normalized to H2afz, used as internal control. The mRNA levels of the 1-cell embryos were defined as 1. Data are expressed as means±s.e.m. (n=3). (B) Localization of CHD1 during mouse preimplantation development (red, CHD1; blue, chromatin). Representative images of embryos at each stage are shown. Between 20 and 50 embryos were used in each experiment (n=3): in total, 103, 96, 117, 79, 81 and 124 embryos each for 1-cell, 2-cell, 4-cell, 8-cell, morula and blastocyst stage, respectively. Shinnosuke Suzuki et al. Development 2015;142: © Published by The Company of Biologists Ltd
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