1. Conversion of proBDNF to mature BDNF occurs after the first pairing session of conditioning as shown by Western blot analysis.
Conversion of proBDNF to mature BDNF occurs after the first pairing session of conditioning as shown by Western blot analysis. A, Significant expression of mature BDNF occurs after one complete pairing session of conditioning (C1, or after 25 min) compared with conditioning for 15 min (C15) or pseudoconditioning for the same time periods (Ps15, Ps1). Expression of BDNF declines to control values after five pairing sessions (C5, or after ∼4 h). B, Western blots of the 30, 24, and 18 kDa forms of proBDNF and mature BDNF after early conditioning show relatively stable amounts of proBDNF protein and appearance of the mature form at C1. Quantitative data of the ratio of proBDNF to BDNF for the 30 and 24 kDa proBDNF proteins show that significant conversion to the mature form occurs at C1. Data from the 18 kDa protein are similar to the 30 and 24 kDa proteins shown.
Joyce Keifer et al. J. Neurosci. 2009;29:
©2009 by Society for Neuroscience