Presentation is loading. Please wait.

Presentation is loading. Please wait.

Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis 

Published byDorcas Campbell Modified over 5 years ago

Similar presentations

Presentation on theme: "Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis "— Presentation transcript:

1 Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis  Stuart I. Myers, MD, Li Wang, BS, Fang Liu, BS, Lori L. Bartula, BS  Journal of Vascular Surgery  Volume 42, Issue 3, Pages (September 2005) DOI: /j.jvs Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 The effect of suprarenal aortic clamping and reperfusion on heart rate. The heart rate was continuously monitored at basal time period, during 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with either saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as beats per minute and expressed as mean ± SEM. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 The effect of suprarenal aortic clamping and reperfusion on mean blood pressure. The mean blood pressure was continuously monitored at basal time period, during 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with either saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as mm Hg and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. BF, blood flow. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 The effect of suprarenal aortic clamping and reperfusion on renal cortical blood flow. Laser Doppler probes were placed 2 mm into the cortex and blood flow (BF) was continuously monitored at basal time period, during 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with either saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as the percentage of change from basal time zero and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 The effect of suprarenal aortic clamping and reperfusion on renal medullary blood flow. Laser Doppler probes were placed 4 mm into the medulla and blood flow (BF) was continuously monitored at basal time period, during 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as the percentage of change from basal time zero and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 The effect of suprarenal aortic clamping and reperfusion on renal cortical nitric oxide (NO) release. Microdialysis probes were placed 2 mm into the cortex, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer solution. Dialysate fluid was collected at basal time zero, following 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, NO synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, NO precursor, N = 7). Data are reported as μmol/L and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

7 Fig 6 The effect of suprarenal aortic clamping and reperfusion on renal medullary nitric oxide (NO) release. Microdialysis probes were placed 4 mm into the medulla, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer solution. Dialysate fluid was collected at basal time zero, following 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, NO precursor, N = 7). Data are reported as μmol/L and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

8 Fig 7 The effect of suprarenal aortic clamping and reperfusion on renal cortical prostaglandin E2 (E2) release. Microdialysis probes were placed 2 mm into the cortex, connected to a syringe pump, and in vivo at 3 μL/min with lactated Ringer solution. Dialysate fluid was collected at basal time zero, following 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as pg/mL PGE2 release and is expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

9 Fig 8 The effect of suprarenal aortic clamping and reperfusion on renal medullary prostaglandin E2 (E2) release. Microdialysis probes were placed 2 mm into the cortex, connected to a syringe pump, and in vivo at 3 μL/min with lactated Ringer solution. Dialysate fluid was collected at basal time zero, following 30 minutes of suprarenal aortic clamping (ischemia) followed by 60 minutes of reperfusion and compared to a sham operation. Both groups were treated with saline carrier (ischemia/reperfusion [IR] group, N = 10), indomethacin (Indo) (10 mg/kg [IR + Indo, N = 9], cyclooxygenase inhibitor), NG-nitro-l-arginine methyl ester (L-NAME) (20 mg/kg, nitric oxide synthase inhibitor, N = 5), or l-arginine (L-Arg) (200 mg/kg, nitric oxide precursor, N = 7). Data are reported as pg/mL PGE2 release and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham; b indicates significance at P < .05 compared to the IR group without drug treatment; c indicates significance at P < .05 compared to the L-NAME group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

10 Fig 9 The effect of suprarenal aortic clamping and reperfusion on renal cortical and medullary inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) content. Protein from renal cortex and medulla was prepared from sham and suprarenal aortic clamping and reperfusion animals and subjected to Western blot analysis to identify content of iNOS and COX-2. The blots were analyzed by densitometry. Data are expressed as densitometry units ± SEM (N = 8). a indicates significance at P < .05 compared to sham. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

11 Fig 10 The effect of suprarenal aortic clamping and reperfusion on creatinine clearance. Serum and urine were collected from separate groups of animals to measure serum creatinine, urine creatinine, and urine volume to calculate creatinine clearance. The serum and urine were collected at the time zero of each experiment and at the end of reperfusion. These groups underwent the same above the superior mesenteric artery aortic clamping (and sham) followed by reperfusion without drug treatment (ischemia/reperfusion [IR], N = 8), treatment with indomethacin (Indo) (IR + Indo, N = 5), treatment with NG-nitro-l-arginine methyl ester (L-NAME) (IR + L-NAME, N = 6), and treatment with l-arginine (L-Arg) (IR + L-Arg, N = 5). Data are reported as mL/min and expressed as mean ± SEM. a indicates significance at P < .05 compared to sham. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Download ppt "Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis "

Similar presentations

Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion  Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,

Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion  Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,
Inhibition of inducible nitric oxide synthase after myocardial ischemia increases coronary flow  Patrick E Parrino, MD, Victor E Laubach, PhD, John R.

Inhibition of inducible nitric oxide synthase after myocardial ischemia increases coronary flow  Patrick E Parrino, MD, Victor E Laubach, PhD, John R.
Volume 63, Issue 2, Pages (February 2003)

Volume 63, Issue 2, Pages (February 2003)
Volume 59, Issue 3, Pages (March 2001)

Volume 59, Issue 3, Pages (March 2001)
Loss of renal function and microvascular blood flow after suprarenal aortic clamping and reperfusion (SPACR) above the superior mesenteric artery is greatly.

Loss of renal function and microvascular blood flow after suprarenal aortic clamping and reperfusion (SPACR) above the superior mesenteric artery is greatly.
The impact of selective visceral perfusion on intestinal macrohemodynamics and microhemodynamics in a porcine model of thoracic aortic cross-clamping 

The impact of selective visceral perfusion on intestinal macrohemodynamics and microhemodynamics in a porcine model of thoracic aortic cross-clamping 
Cyril H. Barton, Zehnmin Ni, Nosratola D. Vaziri  Kidney International 

Cyril H. Barton, Zehnmin Ni, Nosratola D. Vaziri  Kidney International 
Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia- reperfusion injury  Rachel J. Santora, MD, Mihaela L. Lie, MD, Dmitry.

Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia- reperfusion injury  Rachel J. Santora, MD, Mihaela L. Lie, MD, Dmitry.
Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,
Volume 80, Issue 8, Pages (October 2011)

Volume 80, Issue 8, Pages (October 2011)
Repetitive progressive thermal preconditioning hinders thrombosis by reinforcing phosphatidylinositol 3-kinase/Akt-dependent heat-shock protein/endothelial.

Repetitive progressive thermal preconditioning hinders thrombosis by reinforcing phosphatidylinositol 3-kinase/Akt-dependent heat-shock protein/endothelial.
Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion  Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,

Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion  Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,
Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes  N. Chabane, M.Sc.,

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes  N. Chabane, M.Sc.,
Volume 64, Issue 5, Pages (November 2003)

Volume 64, Issue 5, Pages (November 2003)
Volume 63, Issue 3, Pages (March 2003)

Volume 63, Issue 3, Pages (March 2003)
Selective renal blood perfusion induces renal tubules injury in a porcine model  Johannes Kalder, MD, Maria Kokozidou, DVM, MSc, PhD, Paula Keschenau,

Selective renal blood perfusion induces renal tubules injury in a porcine model  Johannes Kalder, MD, Maria Kokozidou, DVM, MSc, PhD, Paula Keschenau,
John C. Kerr, B. S. , Krishna M. Jain, M. D. , Kenneth G. Swan, M. D

John C. Kerr, B. S. , Krishna M. Jain, M. D. , Kenneth G. Swan, M. D
Cerebral metabolic recovery from deep hypothermic circulatory arrest after treatment with arginine and nitro-arginine methyl ester  Takeshi Hiramatsu,

Cerebral metabolic recovery from deep hypothermic circulatory arrest after treatment with arginine and nitro-arginine methyl ester  Takeshi Hiramatsu,
Nitric oxide–generating β-adrenergic blocker nipradilol preserves postischemic cardiac function  Hitoshi Horimoto, MD, Adam E Saltman, MD, PhD, Glenn.

Nitric oxide–generating β-adrenergic blocker nipradilol preserves postischemic cardiac function  Hitoshi Horimoto, MD, Adam E Saltman, MD, PhD, Glenn.
Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis  Stuart.

Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis  Stuart.

Similar presentations

Ads by Google