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Allergy to human seminal fluid: Cross-reactivity with dog dander

Published byYohanes Iskandar Modified over 5 years ago

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Presentation on theme: "Allergy to human seminal fluid: Cross-reactivity with dog dander"— Presentation transcript:

1 Allergy to human seminal fluid: Cross-reactivity with dog dander
Maria Basagaña, MD, Borja Bartolomé, MD, PhD, Carlos Pastor, MD, PhD, Ferran Torres, MD, PhD, Rosario Alonso, MD, Fernando Vivanco, MD, PhD, Anna Cisteró-Bahíma, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 121, Issue 1, Pages (January 2008) DOI: /j.jaci Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, SDS-PAGE after Coomassie Brilliant Blue R-250 staining: lane A, HSP; lane M, molecular mass marker. B, IgE immunoblotting: lane P, patient serum; lane C, control serum (pool from nonatopic subject sera). The + and − symbols indicate the presence and absence of β-mercaptoethanol, respectively. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IgE immunoblotting: lanes 1–41, patient sera; lane C, control serum (pool from nonatopic subject sera); lane M, molecular mass markers. Samples are without β-mercaptoethanol. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 IgE immunoblotting inhibition: lane C, control serum (pool from nonatopic subject sera); lane 1, patient serum; lane 2, patient serum previously incubated with HSP; lane 3, patient serum previously incubated with dog dander extract; lane 4, patient serum previously incubated with lamb extract; lane M, molecular mass markers. Samples are without β-mercaptoethanol. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Identification by means of MS. Peptide fingerprinting of the 28-kd protein is shown. The signaled peaks were analyzed by means of MS/MS, obtaining the amino acid sequence. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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