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The NFκB inhibitory peptide, IκBα, prevents human vascular smooth muscle proliferation
Craig H Selzman, MD, Brian D Shames, MD, Robert C McIntyre, MD, Anirban Banerjee, PhD, Alden H Harken, MD The Annals of Thoracic Surgery Volume 67, Issue 5, Pages (May 1999) DOI: /S (99)
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Fig 1 Growth factor and cytokine-induced vascular smooth muscle proliferation. VSMCs were incubated in control media (Cont) or increasing concentrations of FGF (A) or TNF (B) for 24 h. Both FGF and TNF induced concentration-dependent VSMC (∗p < versus control). The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 2 Influence of nonspecific and specific NFκB blockade on unstimulated VSMC growth. Dexamethasone (Dex), calpain inhibitor-1 (CI-1), liposomes containing the GST moiety alone (Lipo), and liposomal IκBα (IκBα) had no effect on unstimulated VSMC proliferation. The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 3 Influence of nonspecific NFκB inhibition on mitogen-stimulated VSMC. FGF (10 ng/mL) and TNF (10 ng/mL) induced VSMC proliferation compared with control (†p < 0.001). Both dexamethasone (Dex) and calpain inhibitor-1 (CI-1) inhibit FGF- and TNF-induced VSMC proliferation (∗p < 0.001, versus [A] FGF and [B] TNF). The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 4 Influence of specific NFκB blockade with liposomal delivery of IκBα on mitogen-stimulated VSMC. Both FGF (10 ng/mL) and TNF (10 ng/mL) induced VSMC proliferation compared with control (†p < 0.001). Simultaneous incubation with liposome containing the GST moiety alone (Lipo) had no effect on FGF- or TNF-induced proliferation. Liposomal delivery of IκBα (IκBα) inhibited both FGF- and TNF-stimulated VSMC proliferation (∗p < 0.001, versus [A] FGF and [B] TNF). The Annals of Thoracic Surgery , DOI: ( /S (99) )
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