1. KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules.
KIF13A overexpressionfavorsNP distribution to the cell edge and requires KIF13A binding to microtubules. (A-C) HeLa cells were transfected with GFP (A) GFP-KIF13A-WT (GFP-KIF13A) (B) or GFP-KIF13A-ST (GFP-KIF-ST) (C). At 24 h post transfection cells were infected (or mock-infected) with PR8 at an MOI=3. After 8 hpi cells were fixed and stained for NP (red), endogenous Rab11 (gray) and counterstained with DAPI (scale bars: 10 µm). Boxed areas highlight the proximity between KIF13A and vRNP-Rab11 vesicles and are magnified to the right of these panels. (D) Sketch of KIF13A wild-type (WT) and mutant (KIF13A-ST) forms. (E) The amount of NP at the edge of the cell was quantified in percent by calculating the ratio of NP intensity within 2 μm from the plasma membrane to the NP intensity of the whole cell. Data obtained of 25 cells and three replicates were plotted and analysed statistically by using one-way ANOVA followed by Tukey's multiple comparisons, and were considered significant at **P<0.01 and ***P<0.001.
Ana Ramos-Nascimento et al. J Cell Sci 2017;130:
© Published by The Company of Biologists Ltd