1. Mitochondria are uniformly distributed along the axon.
Mitochondria are uniformly distributed along the axon. (A) Distribution of Mitotracker-labeled mitochondria after 1 minute from a time-lapse movie. (B) Kymograph of the movie: horizontal bar=10 μm, vertical bar=1 minute. (C) Color-inverted image of (A) with height stretched by a factor of five in order to resolve the individual mitochondria better. (A-D) Brackets show a cluster of four mitochondria that can be resolved by analysis of the kymograph but cannot be resolved in the still picture. (D) Estimated centers of individual mitochondria, used for statistical analysis. (E) Intensity plot of the mitochondria, with arrows showing divisions between peaks. (F) Sum of the area for each of the peaks shown in (E). (G) Peak size vs estimated number of mitochondria. (H) Frequency distribution from the χ2 analysis of the goodness of fit of the predicted Poisson distribution vs the observed distribution.
Kyle E. Miller, and Michael P. Sheetz J Cell Sci 2004;117:
© The Company of Biologists Limited 2004