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Fig. 2. Coupling of actin to cell–cell junctions requires α-catenin and is necessary for the establishment of the barrier.(A) Effect of Cytochalasin D on barrier formation in MDCK cells. Coupling of actin to cell–cell junctions requires α-catenin and is necessary for the establishment of the barrier.(A) Effect of Cytochalasin D on barrier formation in MDCK cells. CytoD was added during a calcium switch (dashed green line) or to a pre-existing monolayer (dark green line). Error bars indicate standard error, n>3. (B) Schematic representation of α-catenin-WT construct and α-catenin mutant lacking the C-terminal F-actin binding site. (C) Expression levels of α-catenin-WT or α-catenin in α-catenin depleted MDCK cells assessed by Western Blotting with α-catenin specific antibodies. (D) TER measurement in α-catenin depleted MDCK cells (red line), or the same cells rescued with α-catenin-WT (green line) or α-catenin (orange line). Error bars indicate standard error, n>3. Floor Twiss et al. Biology Open 2012;bio © Published by The Company of Biologists Ltd
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