1. Extended analysis of differential expression datasets.
Extended analysis of differential expression datasets. (A) Heatmap of all quantifiable genes in the dataset demonstrates good clustering by biological replicates. TO, total mRNA; TR, translated mRNA. (B) GSEA comparing the list of genes down-regulated (left) or up-regulated (right) transcriptionally under DR twofold or more in the present study against the ordered gene set regulated because of intermittent fasting (Honjoh et al, 2009). The top enrichment profile shows the cumulative enrichment score as each ranked gene in the list is compared with the gene set. Where each gene in the list falls in the gene set is represented by black bars in the middle section. The bottom of the plot depicts the ranked list metric which measures a gene's correlation with the treatment. Both lists were significantly (FDR < 0.001) enriched as compared with the microarray. Better agreement to intermittent fasting was found among the down-regulated genes (normalized enrichment score = −3.25) than up-regulated genes (normalized enrichment score = −2.10). More details are available in the Materials and Methods section.
Jarod A Rollins et al. LSA 2019;2:e
© 2019 Rollins et al.