1. Phosphorylation of AMPK-Thr172 before and after metformin treatment in muscle from subjects with type 2 diabetes.
Phosphorylation of AMPK-Thr172 before and after metformin treatment in muscle from subjects with type 2 diabetes. Proteins (60 μg) from the muscle lysates were separated by 8% SDS-PAGE and transferred to nitrocellulose membranes. Blotting was done with the phospho-AMPK-Thr172 antibody described in research design and methods. A representative blot from one subject before and after 10 weeks of metformin treatment is shown. Data are means ± SE; n = 8; *P < 0.05 vs. pretreatment.
Nicolas Musi et al. Diabetes 2002;51:
©2002 by American Diabetes Association