Presentation is loading. Please wait.

Presentation is loading. Please wait.

Joanna J. Lukawska, MD, Lefteris Livieratos, PhD, Barbara M

Published byHugo O’Brien’ Modified over 5 years ago

Similar presentations

Presentation on theme: "Joanna J. Lukawska, MD, Lefteris Livieratos, PhD, Barbara M"— Presentation transcript:

1 Real-time differential tracking of human neutrophil and eosinophil migration in vivo 
Joanna J. Lukawska, MD, Lefteris Livieratos, PhD, Barbara M. Sawyer, Tak Lee, MD, ScD, Michael O'Doherty, MD, MD, Philip J. Blower, PhD, Martin Kofi, James R. Ballinger, PhD, Christopher J. Corrigan, MD, PhD, Gopinath Gnanasegaran, MD, Ehsan Sharif-Paghaleh, PhD, Gregory E.D. Mullen, PhD  Journal of Allergy and Clinical Immunology  Volume 133, Issue 1, Pages e1 (January 2014) DOI: /j.jaci Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Example images (anterior view) of 1-minute frames from the acquired dynamic image sequence shown at 1, 5, 15, 25, and 120 minutes after injection of radiolabeled neutrophils (top row) and eosinophils (bottom row). The lung, liver, and spleen are indicated by arrows. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Percentage mean lung TACs of labeled neutrophils and eosinophils in 4 volunteers with a fitted curve. Error bars represent ± 1 SD, with a fitted curve. Rapid clearance of eosinophils from the left and right lungs was exhibited (mean half-life, 4.16 ± 0.11 minutes). Neutrophils had significantly slower lung clearance (mean half-life, 13.72 ± 4.14 minutes). Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Mean TACs for neutrophils and eosinophils of the liver (A) and spleen (B). Error bars represent ± 1 SD. In the liver eosinophils showed initial faster uptake, a reflection of faster lung migration. There were no significant differences noted after the first 15 minutes. In the spleen eosinophils demonstrated significantly higher uptake than neutrophils (P = .014). Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Example of the distribution of radioactivity over 250 minutes for the lungs, liver, spleen, and mediastinum in a single representative healthy volunteer for purified autologous neutrophils (A) and purified autologous eosinophils (B). cps, Counts per second. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E1 CD16/CD11bhigh neutrophil population established by means of flow cytometry. A, Unfixed healthy volunteer whole blood was stained with CD16 and CD11b and analyzed by means of flow cytometry. Quadrants are based on negatively stained cells and isotype controls. Cells were gated on granulocytes and analyzed for the CD16/CD11blow population (left square gate). B, The CD16/CD11bhigh neutrophil population was determined by means of incubation of unfixed healthy volunteer whole blood with GM-CSF and then analyzed by means of flow cytometry. Quadrants are based on negatively stained cells and isotype controls. Cells were gated on granulocytes and analyzed for the CD16/CD11bhigh population (right square gate). Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Joanna J. Lukawska, MD, Lefteris Livieratos, PhD, Barbara M"

Similar presentations

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes  Annette T. Hastie, PhD, Wendy C. Moore,

Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes  Annette T. Hastie, PhD, Wendy C. Moore,
Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S

Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S
Ventilation defect percent in helium-3 magnetic resonance imaging as a biomarker of severe outcomes in asthma  David G. Mummy, MS, Stanley J. Kruger,

Ventilation defect percent in helium-3 magnetic resonance imaging as a biomarker of severe outcomes in asthma  David G. Mummy, MS, Stanley J. Kruger,
Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 

Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD 

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD 
Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis  Kaori Mukai, PhD, Nicolas Gaudenzio,

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis  Kaori Mukai, PhD, Nicolas Gaudenzio,
Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Desensitization to Chemotherapeutic Agents

Desensitization to Chemotherapeutic Agents
Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease  Paul T. King, MD, PhD, Steven Lim,

Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease  Paul T. King, MD, PhD, Steven Lim,
Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model  Akihiro Hasegawa, PhD, Katsuhiro.

Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model  Akihiro Hasegawa, PhD, Katsuhiro.
The BLNK adaptor protein has a nonredundant role in human B-cell differentiation  Chantal Lagresle-Peyrou, PhD, Michèle Millili, Sonia Luce, Annie Boned,

The BLNK adaptor protein has a nonredundant role in human B-cell differentiation  Chantal Lagresle-Peyrou, PhD, Michèle Millili, Sonia Luce, Annie Boned,

Similar presentations

Ads by Google