1. The complexity and the total dendritic length of ApoE4 adult-born granule cells are significantly reduced.
The complexity and the total dendritic length of ApoE4 adult-born granule cells are significantly reduced. A, B, Representative 3D-reconstructed adult-born granule cells (4 weeks after stereotactic injections), one circle every 10 µm. Representative picture of 4-week-old retrovirally labeled adult-born granule cells in the dentate gyrus. C, Distance between the soma and the first dendritic intersection (in µm). D, Nodes defined as the number of divisions of dendritic branches. E, Cumulative length of dendritic arborizations (in µm). F, Span of the dendritic arborizations when projected on two dimensions. G, Sholl analysis: number of dendritic intersections as a function of the distance from the soma. ApoE3, 75 neurons/6 mice; ApoE4, 69 neurons/4 mice. Results are expressed as the mean ± SEM. Independent t test and two-way ANOVA with uncorrected Fisher’s LSD post hoc tests were used: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < Scale bar, B, 50 µm.
Yacine Tensaouti et al. eNeuro 2018;5:ENEURO
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