1. 7 WT 6
irx1-6
ixr1-2 5 4
fold increased expression 3 2 n- 1
AT1G18710
AT2G39800
AT3G14440
AT4G39950
AT4G26080
AT2G46680
AT5G57050
Supplemental Figure 2: qRT-PCR analysis of genes differentially up- or down-regulated in secondary cell wall irx mutants. RNA samples from uninfected leaves of three weeks old wild type (WT), ixr1-2 and irx1-6 plants were used for the analysis. Values are represented as n-fold increased expression compared to the genotype showing the lowest expression. Data correspond to the average ( standard deviation) of two replicates. The genes analyzed were selected from Supplemental Tables 1 and 2: AT1G18710 (MYB47 Transcriptional Factor (TF)), AT2G39980 (Transferase protein family), AT2G46680 (HB-7 TF), AT2G21650 (MYB TF); AT3G14440 (NCED3), AT4G26080 (ABI1), AT4G39950 (CYP79B2), and AT5G57050 (ABI2).