1. DHA induces the formation of Pex11pβ-rich regions on elongated peroxisomes.
DHA induces the formation of Pex11pβ-rich regions on elongated peroxisomes. (A) AOx-deficient fibroblasts were cultured in the absence (Aa–Ac) or presence (Ad–Ai) of 150 μM DHA for 12 hours and then stained with antibodies to Pex14p (Aa,Ad,Ag) and Pex11pβ (Ab,Ae,Ah); the merged view of the two proteins is shown in Ac, Af and Ai. Insets are higher-magnification images of the boxed regions. Scale bars: 10 μm and 1 μm (insets). Arrowheads indicate regions enriched with Pex11pβ compared with Pex14p. The numbers 1, 2 and 3 correspond to those in panel B. (B) Mutually distinct peaks of Pex11pβ and Pex14p were apparent upon in-line scanning of the transecting line shown in Ag–Ai. The intensity of Pex11pβ signals was significantly increased at regions (arrowheads) that did not contain Pex14p.
Akinori Itoyama et al. J Cell Sci 2012;125:
© 2012.