1. The effect of LGL-1 on myosin accumulation in par-2 is epistatic to par-6(RNAi).
The effect of LGL-1 on myosin accumulation in par-2 is epistatic to par-6(RNAi). (A) Confocal projections of cortical NMY-2::GFP prior to symmetry breaking (left), at pseudocleavage (center) and at nuclear envelope breakdown (NEB) of the first mitotic division (right). (B) The left column shows confocal projections of cortical NMY-2::GFP in embryos at NEB. The column on the right depicts the threshold mask (red overlay) applied to the images on the left to quantify the fraction of the cortex with NMY-2::GFP signal. Anterior is towards the left in all figures. (C) The average fraction of the cortex with NMY-2::GFP signal±s.d. based on the threshold mask exemplified in B. The values for the average fraction of the cortex with signal are provided in Table 1 in the ‘Area method’ column. Asterisks indicate significant differences relative to par-6(RNAi). P<0.002.
Alexander Beatty et al. Development 2013;140:
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