1. Slc7a5 expression in preosteoclasts is reduced in ovariectomized mice.
Slc7a5 expression in preosteoclasts is reduced in ovariectomized mice. (A) C57BL6 mice were subjected to ovariectomy (OVX) or sham operation and subsequent isolation of CD11blow/−CX3CR1+Ly6Chi cells (preosteoclasts) and CD45−CD51+Sca1− cells (osteoblasts) by flow cytometry. (B and C) Quantification of mRNAs encoding amino acid transporters in preosteoclasts (B) and osteoblasts (C) by qPCR. n = 5 mice. All data were analyzed by the two-tailed Student’s t test. **P < 0.01, significantly different from the value obtained for sham-operated mice. U.D., under detectable values.
Kakeru Ozaki et al., Sci. Signal. 2019;12:eaaw3921
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works