1. KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression.
KIF13A depletion leadsto a drop in virus titers, without affecting viral protein expression. A549 cells were treated with non-targeting or KIF13A-specific siRNAs and infected or mock-infected with PR8 at a multiplicity of infection (MOI) of 3. (A) At the indicated time points, supernatants were collected and viral production was evaluated in plaque assays by using MDCK cells. Statistical analysis of data was performed using non-parametric Kolmogorov–Smirnov test, unpaired (*P<0.05). (B) KIF13A expression was evaluated at the level of transcription by RT-qPCR in relation to GAPDH. (C) Viral protein expression of PB1, HA, NA, NP, NS1, M1 and the control GAPDH analysed by western blotting. (D) Expression of viral proteins was quantified and normalized to GAPDH and presented as the percentage of each protein in the non-targeting siRNA condition at 10 h. Data are shown as means±s.e.m. from four independent experiments and statistical significance is indicated as **P<0.01, ***P<0.001 by non-parametric two-way ANOVA tests followed by Tukey multiple comparison test.
Ana Ramos-Nascimento et al. J Cell Sci 2017;130:
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