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Figure 1 Cell gating and binding curve from FACS experiments and M23 and M1 antibody titers during relapses and remission Cell gating for fluorescence-activated.

Published byJanusz Żurawski Modified over 5 years ago

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Presentation on theme: "Figure 1 Cell gating and binding curve from FACS experiments and M23 and M1 antibody titers during relapses and remission Cell gating for fluorescence-activated."— Presentation transcript:

1 Figure 1 Cell gating and binding curve from FACS experiments and M23 and M1 antibody titers during relapses and remission Cell gating for fluorescence-activated cell sorting (FACS) experiments showing (A) gating of human embryonic kidney 293 cells based on cell size and granularity (R1 gate) and (B) gating of M23R cells to differentiate stable dsRed2/aquaporin-4 (AQP4)-expressing cells (R2 gate) from untransfected and poorly expressing cells (R3 gate). Cell gating and binding curve from FACS experiments and M23 and M1 antibody titers during relapses and remission Cell gating for fluorescence-activated cell sorting (FACS) experiments showing (A) gating of human embryonic kidney 293 cells based on cell size and granularity (R1 gate) and (B) gating of M23R cells to differentiate stable dsRed2/aquaporin-4 (AQP4)-expressing cells (R2 gate) from untransfected and poorly expressing cells (R3 gate). (C) Example of a binding curve of serial doubling dilutions of an individual serum. The binding curve was sigmoidal and the endpoint titer was taken as the dilution of the last sample to score above the cutoff. (D) Correlation between FACS and cell-based assay (CBA) endpoint dilution titers. There was a strong correlation between the 2 techniques (r = 0.802; p < ). (E) Relative levels of M23 and M1 antibodies during relapse and remission. Median M23 antibody titers were higher than M1 antibody titers during both relapse (2,560 vs 180; p < ) and remission (1,120 vs 80; p < ). Median M23 antibody titers were significantly higher during relapse than M23 antibody titers during remission (2,560 vs 1,120; p = ). Median M1 antibody titers were also higher during relapse than during remission, but this result did not reach significance (180 vs 80; p = ). (F) Median M23 and M1 antibody titers from all relapse samples were plotted according to anatomical site of attack. Kruskal-Wallis analysis revealed no significant difference in M23 or M1 antibody titers between different attack types (p = ; p = ). Note log scale. FITC = fluorescein isothiocyanate; LETM = longitudinally extensive transverse myelitis; MFI = mean fluorescence intensity; ON = optic neuritis. Joanna Kitley et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e121 © 2015 American Academy of Neurology

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