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Adverse reaction to lupine-fortified pasta

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Presentation on theme: "Adverse reaction to lupine-fortified pasta"— Presentation transcript:

1 Adverse reaction to lupine-fortified pasta
Hefle, PhD, Lemanske, MD, Bush, MD, BC  Journal of Allergy and Clinical Immunology  Volume 94, Issue 2, Pages (August 1994) DOI: /ai.1994.v94.a54942

2 FIG. 1 Lupine RAST assay inhibition by the various lupine extracts and CPE. Diamonds, Lupine seed extract; squares, lupine pasta extract; circles, CPE. Standard deviations for all values were below 4%. Journal of Allergy and Clinical Immunology  , DOI: ( /ai.1994.v94.a54942)

3 FIG. 2 FAST-stained (Zoion Research) SDS-PAGE gel of affinity-purified peanut proteins. Approximately 50 μg of protein was loaded per lane. Lane A contains molecular weight markers. Lane B contains lupine-fortified pasta extract. Lane C contains lupine seed flour extract. Journal of Allergy and Clinical Immunology  , DOI: ( /ai.1994.v94.a54942)

4 FIG. 3 Autoradiograph of an immunoblot of SDS-PAGE–separated lupine proteins with peanut-sensitive IgE. Bound IgE was detected with rabbit-anti-human IgE labeled with 125I. Exposure time was 72 hours. Approximately 25 μg of protein was loaded per lane. Approximate molecular weights are indicated on the left. Lane numbers denote different serum samples; the strips marked C1 and C2 were incubated with sera from control (nonallergic) serum. Journal of Allergy and Clinical Immunology  , DOI: ( /ai.1994.v94.a54942)

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