1. Defects in neuronal and glial process outgrowth
Defects in neuronal and glial process outgrowth. a–c , Sections through the cerebral cortex of P0 animals of the indicated genotype were stained with antibodies to MAP2 (green), to reveal dendrites.
Defects in neuronal and glial process outgrowth. a–c , Sections through the cerebral cortex of P0 animals of the indicated genotype were stained with antibodies to MAP2 (green), to reveal dendrites. Note that dendrite morphology was severely affected in Itgb1-CNSko mice only. d–l , Dissociated cells from the cerebral cortex of P0 animals of the indicated genotype were plated onto PDL/LN substrates and cultured for 1 d (1div) or 5 d (5div). The cultures were stained with antibodies to MAP2 or GFAP (brown), as indicated. d–f , In cultures containing cells from wild-type mice, neurite outgrowth was evident at 1div and more pronounced by 5div. Many glial cells also formed extensive projections (arrows in f ). g–i , Neurite outgrowth and the formation of glial processes was severely impaired in primary cultures containing cells from Itgb1-CNSko mice. j–l , Neurite outgrowth and the formation of glial processes were restored in cultures with cells from Itgb1-NEXko mice. m , n , The length of the glial processes and neurites was determined. Values indicated the mean and SD. A Student's t test was performed (***p < 0.01). Scale bars: a–c , 50 μm; d–l , 76 μm.
Richard Belvindrah et al. J. Neurosci. 2007;27:
©2007 by Society for Neuroscience