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Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development  Byung Chul Jee, M.D., Hai-Ying Chen,

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Presentation on theme: "Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development  Byung Chul Jee, M.D., Hai-Ying Chen,"— Presentation transcript:

1 Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development  Byung Chul Jee, M.D., Hai-Ying Chen, B.Sc., Ri-Cheng Chian, Ph.D.  Fertility and Sterility  Volume 91, Issue 5, Pages (May 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 A schematic diagram of the study. Three doses of cilostamide (1 μmol/L, 5 μmol/L, and 10 μmol/L) were used for prematuration cultures. PMSG = pregnant mare serum gonadotropin; CEO = cumulus-enclosed oocyte; GV = germinal vesicle–stage oocyte; MII = metaphase II oocyte. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Meiotic inhibition efficiencies after prematuration culture (PMC) for 24 hours by cilostamide in mouse cumulus-enclosed oocytes (CEOs). The arrest efficiencies were 86.7%, 100%, and 100% after 24 hours PMC, after treatment with cilostamide at concentrations of 1 μmol/L, 5 μmol/L, and 10 μmol/L, respectively (P=.037). GV = germinal vesicle–stage oocyte. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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