1. Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A), PMSM (B), PMSM containing 10 mM d-serine as the sole source of carbon (C), PMSM containing 10 mM d-serine as the sole source of nitrogen (D), PMSM containing 10 mM l-serine as the sole source of carbon (E), or PMSM containing 10 mM l-serine as the sole source of nitrogen (F), and growth was assessed as described in the legend for Fig. 1.
Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A), PMSM (B), PMSM containing 10 mM d-serine as the sole source of carbon (C), PMSM containing 10 mM d-serine as the sole source of nitrogen (D), PMSM containing 10 mM l-serine as the sole source of carbon (E), or PMSM containing 10 mM l-serine as the sole source of nitrogen (F), and growth was assessed as described in the legend for Fig. 1. Error bars represent means ± standard deviations (SDs) from at least 6 replicates, and graphs are representative of 3 independent experiments. In PMSM with d-serine as the sole source of carbon or nitrogen, the WT exhibited significantly increased growth compared to that of the mutants by paired two-way ANOVA with Dunnett’s test for multiple comparisons (P < 0.001).
Aimee L. Brauer et al. mSphere 2019; doi: /mSphere