1. UNIVERSITY OF COIMBRA
Faculty of Science and Thecnology
Department of Chemistry
Dealing with the (ab)use of antibiotics: hybrid materials for heterogeneous catalytic downstream treatment
Giusi Piccirillo
(PhD student)
Supervisors:
Prof. Mário José Ferreira Calvete
Prof. Maria Ermelinda da Silva Eusébio
Prof. João Paulo C. Tomé
Lisbon, July 2019

2. What is the CQC?
Unit dedicated to research in Chemistry in the University of Coimbra
Research infrastructure of the Department of Chemistry of the University of Coimbra
Involves about 90 integrated scientists with PhD degree, plus many Doctoral, Masters and project students

3. Developing collaborations with industry
Main objectives:
Advancing research
Industry
University
Developing collaborations with industry
Providing high-level advanced training to national and international students

4. What is the real problem?
Fig.1 Global increase and geographic convergence in antibiotic consumption between 2000 and 2015, published March 26, 2018 in the Proceedings of the National Academy of Science (PNAS).
Pharmaceuticals in drinking-water, WHO report, Available at ISBN accessed on
E. Y. Klein, T. P. Van Boeckel, E. M. Martinez, S. Pant, S. Grandra, S. A. Levin, H. Goossens, R. Laxminarayan, PNAS, 2018.
G.Gopalakrishna, J. Knoeff, M. Verhoef, T. Prasad, Antimicrobial Resistance Benchmark 2018 Methodology Report, 2017.

5. How to solve this problem…
Direct sunlight
Homogeneous Processes
Heterogeneous Processes
O. Gonzalez, C. Sans, S. Esplugas, J. Hazard Mater., 2007, 146, 459.
K. Kümmerer, Chemosphere, 2009, 75, 417.
Y. Deng, R. Zhao, Cur. Pollution Rep., 2015, 1, 167.
A. S. Oliveira, E. M. Saggioro, T. Pavesi, J. C. Moreira, L. F. V. Ferreira, Molecular Photochemistry-Various Aspects, INTECH, 2012, 9.

6. Aim of my PhD project
(Photo)degradation
S. M. A. Pinto, C. S. Vinagreiro, V. A. Tomè, G. Piccirillo, L. Damas, M. M. Pereira, JPP, 2019, 23, 1-18.
M. J. F. Calvete, G. Piccirillo, C. S. Vinagreiro, M. M. Pereira, Coord. Chem. Rev., 2019, 395,

7. Oxidation Products Homogeneous Degradation of Trimethoprim (TMP)
Catalytic
Oxidation
Oxidation Products
H2O2
catalyst 2h
Best experimental conditions:
[TMP]=1g/L
[MnTDCPPs]=0,04g/L
[H2O2]final=0,02g/L
catalyst
L. D. Dias, R. M. B. Carrilho, C. A. Henrique, G. Piccirillo, A. Fernandes, L. M. Rossi, M. F. Ribeiro, M. J. F. Calvete, M. M. Pereira, JPP, 2018, 22,

8. HPLC analysis of oxidation products
HPLC-MS analysis
I.S.
TMP
Product
Retention Time
4.089 min
6.625 min
8.628 min
min
I.S.
Figure. Hplc chromatograms of the TMP degradation after 2h.
Method: Phenomenex Luna 5u C18 column (150 x 4.6 mm) reverse phase; 15% CH3OH, 15% CH3CN, 70% CH3COONH4 10mM (pH 9.52) (as mobile phase); flux mL min-1; λ=270,8 nm.
In collaboration with Prof. Artur Silva and
Dr. Monica Válega (University of Aveiro)

9. Heterogeneous Degradation of Trimethoprim (TMP)

10. Reutilization test are ongoing
Heterogeneous Degradation of Trimethoprim (TMP)
Catalytic
Oxidation
Same oxidation Products
H2O2
catalyst 2h
Reutilization test are ongoing
catalyst

11. Efficient system for TMP
Conclusions
Efficient system for TMP
catalytic oxidation +
H2O2
Aknowledgments