1. Localization of wild-type and mutant FHOD1 at cellular actin structures.
Localization of wild-type and mutant FHOD1 at cellular actin structures. Two cell systems were used to study the subcellular localization of FHOD1. (A) Neuro2a cells expressing constitutively active Rac1 form extensive lamellipodia, filopodia and actin arcs. (B) COS7 cells also form ventral stress fibers. If expressed at very low levels using a truncated CMV promoter (ΔCMV), wild-type FHOD1 speckles localize to various actin-rich structures including filopodia and actin arcs in Neuro2a cells and on a subset of stress fibers in COS7 cells (cyan arrows). Constitutively active FHOD1 ΔC is enriched on actin arcs in Neuro2a cells and strongly localized to stress fibers in COS7 cells. The N-terminal region of FHOD1 1–573 is both necessary and sufficient for its localization to actin stress fibers, while FHOD1 ΔN is largely cytosolic. Wild-type FHOD1, as well as its ΔC variant and the 1–573 regions are largely excluded from actin-rich focal adhesions (cyan arrowheads). The corresponding FHOD1 image panels within each cell type were all acquired and scaled with identical settings to allow comparison of expression level. Insets are magnified 3× from original panels and optimized individually for display purposes.
André Schönichen et al. J Cell Sci 2013;126:
© Published by The Company of Biologists Ltd