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Ozone activates pulmonary dendritic cells and promotes allergic sensitization through a Toll-like receptor 4–dependent mechanism  John W. Hollingsworth,

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Presentation on theme: "Ozone activates pulmonary dendritic cells and promotes allergic sensitization through a Toll-like receptor 4–dependent mechanism  John W. Hollingsworth,"— Presentation transcript:

1 Ozone activates pulmonary dendritic cells and promotes allergic sensitization through a Toll-like receptor 4–dependent mechanism  John W. Hollingsworth, MD, Meghan E. Free, BSc, Zhuowei Li, MD, Laura Novack Andrews, BSc, Hideki Nakano, PhD, Donald N. Cook, PhD  Journal of Allergy and Clinical Immunology  Volume 125, Issue 5, Pages (May 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Ozone functions as a TLR4-dependent adjuvant in the airway. A, Timeline. Mice were sensitized at the indicated times by means of airway delivery of OVA together with LPS or immediately after exposure of the mice to either ozone or filtered air (air). Two days after challenge with aerosolized OVA, mice were killed, blood was collected for measures of total IgE, and bronchoalveolar lavage was performed. Leukocyte recruitment to the airway (B), total serum IgE levels (C), and cytokines in lung homogenates of wild-type (solid rectangles) or tlr4-deficient (open rectangles) mice (D) after allergic sensitization and challenge are shown. Data shown are from one of 3 experiments yielding similar results. SEs are shown. ∗P < .05, Student t test (n = 5 per group). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Ozone increases CD86 expression on LN DCs. OVA-AF was delivered to recipient mice together with 0.1 μg of LPS or after exposure of the mice to ozone or filtered air. DCs in thoracic LNs were identified as live nonautofluorescent cells displaying the cell-surface marker CD11c (A). OVA-AF+ DCs were further identified as a subset of DCs within the CD11c+ gate (B). OVA-AF+ DCs, presented as total cell number and as a percentage of total LN cells, are shown for each OVA-AF treatment group (C). OVA-AF+ DCs that also stained with antibodies against the indicated costimulatory molecules are presented as a percentage of total LN cells (D). Display levels (mean fluorescence intensity [MFI]) of individual costimulatory molecules are also shown. Data are from one of 2 experiments yielding similar results. SEs are indicated. ∗P < .05, Student t test (n = 7 per group). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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