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Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression.
Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression. HDAC9 mRNA (A) and protein (B) expression were quantified by qPCR and Western blot analyses, respectively, on the indicated days of differentiation. Values represent percentage of change from preadipocytes (0 day) and are expressed as mean ± SEM of three different experiments. C: Western blot images were analyzed utilizing ImageJ software and expressed as percentage of 0 day levels in each condition after normalization to corresponding glyceraldehyde-3-phosphate dehydrogenase values. *P < CD, chow diet; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Tapan K. Chatterjee et al. Diabetes 2014;63: ©2014 by American Diabetes Association
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