1. PEDF blockage improved the reduced numbers of circulating CD34+/CD133+/Flk-1+ cells and impaired EPC functions, including migration, adhesion, and tube formation of diabetic mice.
PEDF blockage improved the reduced numbers of circulating CD34+/CD133+/Flk-1+ cells and impaired EPC functions, including migration, adhesion, and tube formation of diabetic mice. A–D: Representative FACS results of peripheral blood are shown. E–H: Quantification of CD34+/Flk-1+ and CD133+/Flk-1+ cells in peripheral blood (mean ± SD, n = 10 animals/group, *P < 0.05, **P < 0.01). db/db+IgG vs. db/db+PEDF Ab (E), NDM+GST vs. NDM+PEDF (F), PEDF KO vs. WT (G), PEDF KO (DM) vs. WT (DM) (H). I: Representative in vitro migration results are shown. J–L: Migration cell numbers (representative cohort of n = 5 animals/group, **P < 0.01). M: Representative in vitro adhesion results are shown. N–P: Adhesion assay cell numbers (representative cohort of n = 5 animals/group, *P < 0.05, **P < 0.01). Q: Representative in vitro tube formation results are shown. R–T: Tube formation branch point counting (representative cohort of n = 5 animals/group, **P < 0.01). Statistical significance was considered at P < All data were expressed as the mean ± SD.
Weiwei Qi et al. Diabetes 2015;64:
©2015 by American Diabetes Association