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Biju Thomas, MD, Robert Anthony Hirst, PhD, Mina H

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1 Severe asthma: Differential chemokine response of airway epithelial cells 
Biju Thomas, MD, Robert Anthony Hirst, PhD, Mina H. Brett-Pitt, PhD, Gwyneth Williams, CBiol, Peter W. Andrew, PhD, Ana R. Sousa, PhD, Richard P. Marshall, PhD, Chrostopher Brightling, PhD, Christopher O'Callaghan, PhD  Journal of Allergy and Clinical Immunology  Volume 140, Issue 4, Pages e10 (October 2017) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Release of CXCL8 (A and B), CCL11 (C and D), and CCL26 (E and F) by primary respiratory basal cells of patients with severe asthma and healthy control subjects in response to S pneumoniae (D39) at 106 and 107 cfu/mL. Fig 1, A, C, and E, CXCL8 response of basal cells at 1 hour after exposure. Fig 1, B, D, and F, CXCL8 response of basal cells at 4 hours after exposure. Data are expressed as medians (interquartile ranges). †P < .01 compared with corresponding values for severe asthma. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Release of CXCL8 (A and B), CCL11 (C and D), and CCL26 (E and F) by primary respiratory basal cells of patients with severe asthma and healthy control subjects in response to LoTox Der p 1 at 1 and 5 μg/mL. Fig 2, A, C, and E, Response of basal cells at 8 hours after exposure. Fig 2, B, D, and F, Response of basal cells at 24 hours after exposure. Data are expressed as medians (interquartile ranges). †P < .01 compared with corresponding values for healthy control subjects. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Release of IL-1β (A) and IL-6 (B) by primary respiratory basal cells of patients with severe asthma and healthy control subjects in response to S pneumoniae (D39) at 106 and 107 cfu/mL 4 hours after exposure. Release of IL-1β (C) and IL-6 (D) by primary respiratory basal cells of patients with severe asthma and healthy control subjects in response to LoTox Der p 1 at 1 and 5 μg/mL 24 hours after exposure is shown. Data are expressed as medians (interquartile ranges). †P < .01 compared with corresponding values for healthy control subjects. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Light microscopic image of human bronchial epithelial cells in culture. Scale bar = 20 μm. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Immunofluorescent images showing expression of CK14 in basal respiratory epithelial cells. A, Basal cells stained with anti-CK14 antibody (red). B, Negative control showing basal cells only stained with the secondary antibody. Nuclei are stained with Hoechst (blue). Original magnification = ×600. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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