1. Lipopolysaccharide-promoted proliferation of endometriotic stromal cells via induction of tumor necrosis factor α and interleukin-8 expression 
Yumiko Iba, M.D., Tasuku Harada, M.D., Sayako Horie, M.D., Imari Deura, M.D., Tomio Iwabe, M.D., Naoki Terakawa, M.D. 
Fertility and Sterility 
Volume 82, Pages (October 2004)
DOI: /j.fertnstert
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
TLR4 expression in ESC. A, RT-PCR revealed TLR4 mRNA expression. The U937 cell lines were used as a positive control for TLR4. B1, Immunocytochemical staining revealed TLR4 protein expression. B2, Negative control (×400).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
LPS stimulation of TNFα and IL-8 protein production in ESC. TNFα and IL-8 production were increased by adding LPS (0–10 ng/mL) in a dose-dependent manner. Concentrations of TNFα and IL-8 in the supernatants were measured by ELISA. The red bars show TNFα, and the blue bars show IL-8. Bars represent SEs. The means and SEs were calculated from different patients' ESC cultures (n = 8). The cells were used for experiments during 2- to 4-cell passages. *P<.05 vs. controls.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
LPS stimulation of TNFα and IL-8 protein production in ESC. TNFα and IL-8 production was increased by adding LPS (10 ng/mL) in a time-dependent manner up to 24 hours. The red bars show TNFα, and the blue bars show IL-8. Bars represent SEs. The means and SEs were calculated from different patients' ESC cultures (n = 8). The cells were used for experiments during 2- to 4-cell passages. *P<.05 vs. controls.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Immunocytochemical staining of TNFα of ESC. A, Only faint staining was observed when the cells were treated without LPS stimulation. B, Strong staining of ESC after LPS stimulation (×400).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

6. FIGURE 5
Effect of LPS on ESC proliferation. Cell proliferation was determined spectrophotometrically by the incorporation of tetrazolium dye for 72 hours. Results were expressed as a percentage of control values. Anti-TNFα antibody (20 ng/mL) and anti-IL-8 antibody (50 ng/mL) were added to the culture medium containing LPS (10 ng/mL). The red bars show control and LPS, the blue bar shows anti-TNFα antibody, and the yellow bar shows anti-IL-8 antibody. Bars represent SEs. The means and SEs were calculated from different patients' ESC cultures (n = 5). The cells were used for experiments during 2- to 4-cell passages. *P<.05 vs. controls. **P<.05 vs. 10 ng/mL LPS.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

7. FIGURE 6
Effect of IL-8 antisense oligonucleotides on IL-8 production and ESC proliferation. A, IL-8 concentration in the supernatants was measured by ELISA. IL-8 antisense (AS) and scrambled (S) oligonucleotides (2 μM) were added to the culture medium containing LPS (10 ng/mL). B, BrdU incorporation assay was performed. IL-8 antisense (AS) (yellow bar) and scrambled (S) oligonucleotides (2 μM) were added to the culture medium containing LPS (10 ng/mL). The red bars show control and LPS, the blue bar shows IL-8 S, and the yellow bar shows IL-8 AS. Bars represent SEs. The means and SEs were calculated from different patients' ESC cultures (n = 3). The cells were used for experiments during 2- to 4-cell passages. *P<.05 vs. IL-8 S.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

8. FIGURE 7
Effect of the NF-κB inhibitor, TPCK, on IL-8 production and ESC proliferation. A, IL-8 concentration in the supernatants was measured by ELISA. TPCK (10−5 M) was added to the culture medium containing LPS (10 ng/mL). B, Cell proliferation was determined by BrdU incorporation. The red bars show control and LPS, and the yellow bar shows TPCK. Bars represent SEs. The means and SEs were calculated from different patients' ESC cultures (n = 7). The cells were used for experiments during 2- to 4-cell passages. *P<0.05 vs. LPS (10 ng/mL). **P<.05 vs. controls.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions