Presentation is loading. Please wait.

Presentation is loading. Please wait.

Small molecule suppression of ATXN3-CAG89 aggregation.

Published byChristal Brown Modified over 5 years ago

Similar presentations

Presentation on theme: "Small molecule suppression of ATXN3-CAG89 aggregation."— Presentation transcript:

1 Small molecule suppression of ATXN3-CAG89 aggregation.
Small molecule suppression of ATXN3-CAG89 aggregation. (A) Fixed whole ATXN3-CAG10 and ATXN3-CAG89 transgenic worms stained with a human ATXN3 antibody (blue) and TOPRO dye (yellow) for the nucleic acids. Arrows indicate ATXN3 protein in the motoneurons of ATXN3 transgenics. Second panel is a colour-inverted image to aid visualization. The insets are magnifications of the aggregates indicated in the transgenic worms with a dashed line box. (B) Total protein levels for N2 and transgenic worms expressing ATXN3-CAG10 or mutant ATXN3-CAG89. Antibody detection revealed high accumulation of the ATXN3-CAG89 in the pelleted, insoluble fraction, when compared with ATXN3-CAG10 and N2 wild-type worms. There was a reduction of aggregation in ATXN3-CAG89 worms when treated with 60 μM MB, 50 μM Sal or 50 μM Gua. Gua was especially potent in reducing protein aggregation. (C) Quantification of the intensity of the aggregation in transgenic ATXN3-CAG89 mutants and wild-type N2 and ATXN3-CAG10 worms. There was a significant increase of aggregation in ATXN3-CAG89 when compared with the wild-type N2 and ATXN3-CAG10 worms. After treatment with 60 μM MB (*P<0.05), 50 µM Sal (*P<0.05) or 50 µM Gua (***P<0.001) (by ANOVA) ATXN3-CAG89 showed a significant decrease in aggregation levels. Gua, guanabenz; MB, Methylene Blue; Sal, salubrinal. Yasmin Fardghassemi et al. Dis. Model. Mech. 2017;10: © Published by The Company of Biologists Ltd

Download ppt "Small molecule suppression of ATXN3-CAG89 aggregation."

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Mitochondria localize to both the front and the rear of neutrophils.
Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.

Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.
BDNF expression in the cerebellum and brain stem region.

BDNF expression in the cerebellum and brain stem region.
Effect of cxcr3.2 mutation on dissemination of local mycobacterial infection within 24 hpi. Effect of cxcr3.2 mutation on dissemination of local mycobacterial.

Effect of cxcr3.2 mutation on dissemination of local mycobacterial infection within 24 hpi. Effect of cxcr3.2 mutation on dissemination of local mycobacterial.
Inducible DM1 model displays increased autophagy.

Inducible DM1 model displays increased autophagy.
mTOR downregulation, but not autophagy, reduces VAP(P58S) aggregation.

mTOR downregulation, but not autophagy, reduces VAP(P58S) aggregation.
Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.
Arterial Notch signaling is not required for artery patterning.

Arterial Notch signaling is not required for artery patterning.
Fig. 1. Chlamydia infection causes elevated levels of sortilin.

Fig. 1. Chlamydia infection causes elevated levels of sortilin.
Fig. 2. Proportion of motile objects and track length

Fig. 2. Proportion of motile objects and track length
A Drosophila cell culture model to study VAP(P58S) aggregation.

A Drosophila cell culture model to study VAP(P58S) aggregation.
Tomoregulin-1 improves cardiac pathological changes after pressure overload. Tomoregulin-1 improves cardiac pathological changes after pressure overload.

Tomoregulin-1 improves cardiac pathological changes after pressure overload. Tomoregulin-1 improves cardiac pathological changes after pressure overload.
Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.
Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.
Effect of OLHHA on the protein expression of relevant enzymes and factors related to lipid metabolism in the liver of obese Zucker rats. Effect of OLHHA.

Effect of OLHHA on the protein expression of relevant enzymes and factors related to lipid metabolism in the liver of obese Zucker rats. Effect of OLHHA.
TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.
Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.
Scanning EM shows cilia abnormalities in the neural tube.

Scanning EM shows cilia abnormalities in the neural tube.
Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.
Table 2. Cell surface abundance of β1 integrin measured by flow cytometry.DDR wild type and DDR over-expressing cells were treated with deoxymannojirimycin.

Table 2. Cell surface abundance of β1 integrin measured by flow cytometry.DDR wild type and DDR over-expressing cells were treated with deoxymannojirimycin.

Similar presentations

Ads by Google