1. Volume 58, Issue 1, Pages 137-143 (July 2000)
Podocyte cell cycle regulation and proliferation in collapsing glomerulopathies 
Laura Barisoni, Michele Mokrzycki, Leonada Sablay, Michio Nagata, Harold Yamase, Peter Mundel 
Kidney International 
Volume 58, Issue 1, Pages (July 2000)
DOI: /j x
Copyright © 2000 International Society of Nephrology Terms and Conditions

2. Figure 1
Glomerular morphology in HIV-associated nephropathy (HIVAN). The glomerular lesions vary from focal (a) to global (b). (a) The glomerular tuft is segmentally collapsed (asterisks). The glomerular capillaries are partially obliterated, and no extracellular matrix increase is seen. The hypertrophied podocyte cell bodies are prominent, particularly in the area of collapse. (b) In more advanced lesions, the collapse is global involving the entire glomerular tuft. The GBM is folded and wrinkled leading to an increase of Bowman's space, which is filled with hypertrophic and hyperplastic podocytes containing protein reabsorption droplets (arrowheads). (c) At higher magnification, details of the altered podocyte morphology become obvious: Podocytes assume a cuboidal shape and form a “crown” lining up along the outer aspect of the collapsed GBM. The urinary space contains proteinaceous material. Silver staining. (a and b ×100, c ×200).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 2
Podocyte phenotype in normal adult kidney and in early lesions of HIVAN. (Left panels; a–c) Normal adult kidney. Synaptopodin is strongly expressed in normal adult podocytes, at the level of foot processes (a). Nuclear expression of cyclin D1 (b) and p57 (c) is evident in podocyte. (Middle panels; d–f) CG, nonaffected glomerulus. Synaptopodin expression is markedly reduced in nonaffected glomeruli of CG (d) with a weak punctuated staining compared with a strong linear staining seen in normal control (a). Cyclin D1 (e) expression is loss in dysregulated podocytes of nonaffected glomeruli where nuclear immunostaining for p57 is still present (f). (Right panels; g–i) CG (serial sections), segmental collapse. While immunostaining for synaptopodin (g) and cyclin D1 (h) is diffusely reduced or not detectable, loss of p57 expression (i) is restricted to the area of segmental collapse (arrowheads) but is preserved in nonaffected capillaries of the same glomerulus. (a–c, g–i, ×100; d–f, ×120).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 3
Altered podocyte cell cycle and proliferation in CG. Cyclin kinase inhibitor (CKI) expression including p57 (a) and p27 (b) is diffusely lost in globally affected glomeruli. Note that Bowman's space is completely filled with podocytes. In areas of podocyte crowding, cyclin A (c) and Ki-67 (i) expression is observed in podocytes (arrowheads in c and d; ×120). (a and b) Serial sections. (a–d) HIVAN.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions