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Novel West Nile virus lineage 1a full genome sequences from human cases of infection in north-eastern Italy, 2011 L. Barzon Clinical Microbiology and Infection Volume 18, Issue 12, Pages E541-E544 (December 2012) DOI: / Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 1 Map showing areas in north-eastern Italy where human cases of West Nile virus (WNV) infection were detected in 2008–2011. The sites where fully sequenced WNV human genomes were collected in 2009 and 2011 are indicated with black dots. The site where a WNV lineage 2-positive wild collared dove was collected in 2011 is also indicated in the map with a white dot. Clinical Microbiology and Infection , E541-E544DOI: ( / ) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 2 Phylogenetic trees based on the two West Nile virus full-length genomes sequenced from human specimens, Veneto Region, Italy, The evolutionary history was inferred by using the Maximum Likelihood method based on the Kimura two-parameter model [13]. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the strains analysed. Branches corresponding to partitions reproduced in <50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated strains clustered together in the bootstrap test (1000 replicates) are shown next to the branches (only >80 values). Initial trees for the heuristic search were obtained automatically as follows. When the number of common sites was <100 or less than one-quarter of the total number of sites, the maximum parsimony method was used; otherwise BIONJ method with a Markov Cluster distance matrix was used. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. All positions containing gaps and missing data were eliminated. There were a total of positions in the final dataset. Evolutionary analyses were conducted in MEGA5 [14]. Clinical Microbiology and Infection , E541-E544DOI: ( / ) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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